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96 Z. Rechtsmedizin 1 (1986)

handle is hein.journals/injlegame96 and id is 1 raw text is: Zeitschrift fur Z Rechtsmed (1986) 96:1-10 Rechtsmedizin © Springer-Verlag 1986 Originalarbeiten / Original Works Acute Effects of Carbon Monoxide and Cyanide on Hepatic Mitochondrial Function H. Hattoril, Y. Suzuki' 3, T. Fujimiyat, K. Yamamotol, and M. Ueda1 t Department of Legal Medicine, Faculty of Medicine, Kyoto University, Kyoto, 606, Japan 2 Department of Pathology, Chest Disease Research Institute, Kyoto University, Kyoto, 606, Japan Summary. The effects of carbon monoxide and cyanide on the hepatic redox state and energy charge were investigated. Rats were used for the experi- ment under pentobarbital anesthesia. Immediately after laparotomy, a rat was placed in an animal chamber made of a transparent plastic box and ex- posed to a test gas for 3min. Every test gas was produced in a gas chamber connected to the animal chamber with a flexible tube. HCN was produced from NaCN and H2SO4. In the CO inhalation experiment, various amounts of CO were introduced into the gas chamber. Immediately after an expo- sure, about 2g liver was frozen in situ with a precooled clamp. Oozed blood from the wound surface was sampled. Concentrations of ATP, ADP, AMP, acetoacetate, and p3-hydroxybutyrate in hepatic mitochondria were deter- mined, and the redox state and the energy charge were calculated. For cy- anide as well as CO, significant negative correlations were found between the concentration in the blood and the redox state. The same held true for the energy charge. The redox state showed a slight increase at low concen- trations of both gases; however, thereafter it began to decrease sharply with increases in concentrations. When concentrations of the toxicant in the blood reached certain levels, a kind of turning point, beyond which the re- dox state does not decrease any more, was observed. It was about 40% for HbCO and about 2.0 g/ml for cyanide, and the points seemed to be related to the concentrations, beyond which cells are irreversibly damaged. On the other hand, the energy charge did not change at low concentrations. With an increase in toxicant concentrations, the energy charge decreased drastically. The rate of decrease in the energy charge became higher when blood con- centrations exceeded certain levels. It was about 40% for HbCO and 2.0 pg/ ml for cyanide. The presence of low levels of blood cyanide did not affect the relationship between the energy charge and the HbCO concentration. s Present address: Department of Legal Medicine, Yamaguchi University, School of Medicine, Ube, 755, Japan Offprint requests to: H. Hattori (address see above)

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