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84 Z. Rechtsmedizin 1 (1979-1980)

handle is hein.journals/injlegame84 and id is 1 raw text is: Zeitschrift fir
Z. Rechtsmed. 84, 1-6 (1979)                           Rechtsmedizin
O Springer-Verlag 1979
Originalarbeiten / Original Works
Zur ACP-Typendifferenzierung aus Blutflecken
Nachweisgrenzen und Temperaturabhangigkeit
Bernd Brinkmann und Jurgen Bruns
Institut fur Rechtsmedizin der Universitat Hamburg, Butenfeld 34, D-2000 Hamburg 54,
Bundesrepublik Deutschland
Typing ACP Isoenzymes in Blood Stains
Testlimits and Temperature Dependancy
Summary. Two series of test blood stains of known red-cell acid phos-
phatase types A, AB, AC, B, BC, and C are applied to two different types
of stain carriers (glass and cotton) and stored at different temperature ranges:
(a) 37°C at high air humidity, (b) 22°C, (c) 4°C, and (d) -20°C. Electro-
phoresis is carried out either on cellulose-acetate-foils or on thin agarose
layers. Visualisation of the isoenzyme sites is achieved using umbelliferyl
phosphate as a substrate. The most important results are: Electrophoresis on
Cellogel-foils turns out advantageous compared to agarose since (a) less
stain material is required, (b) reproducibility is rather reliable, and (c) electro-
phoretic separation is clearer. Blood stains on glass are identified after longer
periods of storage than stains on cotton. If stains are stored at room temper-
ature the time limits of demonstration are in the range of 3 to 6 weeks. The
size of the sample necessary for demonstrating varies widely. Approximately
twenty to twenty-five per cent of a blood drop are needed. The method
described is recommended as practicable and reliable.
Key words: Blood stains, ACP-ACP isoenzymes in blood stains
Zusammenfassung. Serien von frisch angelegten Blutspuren auf Glas und auf
Baumwolle - alle sechs Phanotypen der sauren Erythrocytenphosphatase
(acP) - werden verschiedenen Lagerungsbedingungen ausgesetzt: 37 C, hohe
Luftfeuchtigkeit; 20°C, Raumtemperatur; 4°C, Kuhlschrank; -20°C, Tief-
ktihlschrank. Die elektrophoretische Darstellung erfolgt parallel mit Hilfe der
,Cellogel-Elektrophorese sowie mit Hilfe der Agarose-Dunnschicht-Elektro-
phorese. Die Darstellung der Isoenzyme erfolgt mit Hilfe des fluoiogenen
Substrats 4-Methyl-Umbelliferylphosphat.
Die wichtigsten Ergebnisse sind: Es werden zur acP-Typendifferenzierung
nur Bruchteile eines Blutstropfens verwendet. Die Verwendung der ,,Cellogel-
Technik ermoglicht a) geringen Spurensubstanzverbrauch, b) sichere Typen-
differenzierung, c) langere Nachweisgrenzen (im Vergleich zur Agarose-Tech-
nik). Spuren auf Glas lassen sich langer nachweisen als Stoffspuren. Die
ACP=englische Abkurzung fur Acid Phosphatase

0044-3433/79/0084/0001/$1.20

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