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102 Z. Rechtsmedizin 1 (1989)

handle is hein.journals/injlegame102 and id is 1 raw text is: Zeitschrift fur
Z Rechtsmed (1989) 102:1-4                       Rechtsmedizin
© Springer-Verlag 1989
Original works
Determination of methadone in human hair
by radioimmunoassay
S.Balabanoval and H. U. Wolf2
1Department of Forensic Medicine, University of Ulm, Prittwitzstrasse 6, D-7900 Ulm,
Federal Republic of Germany
2Department of Pharmacology and Toxicology, University of Ulm, Oberer Eselsberg,
D-7900 Ulm, Federal Republic of Germany
Summary. The concentrations of methadone in human hair were measured.
The washed hair was cut in 1-mm pieces approximately, then incubated
overnight at 45°C with 0.1 m HCl. The extracts were alcalized by 1 m NaOH
and diluted by phosphate buffer, pH 7.4. The methadone concentrations
were determined by radioimmunoassay. The method is simple, rapid, and
practicable for routine determination.
Key word: Methadone, radioimmunologic determination in hair
Zusammenfassung. Die Konzentration von Methadon im menschlichen
Haar wurde bestimmt. Die gewaschenen Haarproben wurden in ca. 1 mm
lange Stucke geschnitten und mit 0.1 m HCl uber Nacht bei 45°C inkubiert.
Die Extrakte wurden mit 1 m NaOH alkalisiert und mit Phosphat-Puffer pH
7.4 verdiinnt. Die Methadonkonzentrationen wurden mittels Radioimmuno-
assay gemessen. Diese Methode stellt eine einfache und schnelle Routine-
bestimmung dar.
Schlisselwort: Methadon, radioimmunologische Bestimmung im Haar
Introduction
Methadone is a synthetic long-acting analgesic promoted as a treatment in de-
toxification and maintenance programs of heroin addiction (Isbell and Vogell
1949; Dole and Nyswandes 1965). However, methadone use results also in habit
formation (Isbell and Vogell 1949; Symonds 1977; Ettinger et al. 1979). Con-
sequently, the use of methadone represents a serious problem and indicates the
necessity of control. In this study, we describe the determination of methadone
in human hair by radioimmunoassay.
Offprint requests to: S. Balabanova

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