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84 Z. Rechtsmedizin 1 (1979-1980)

handle is hein.journals/injlegame84 and id is 1 raw text is: Zeitschrift fir Z. Rechtsmed. 84, 1-6 (1979) Rechtsmedizin O Springer-Verlag 1979 Originalarbeiten / Original Works Zur ACP-Typendifferenzierung aus Blutflecken Nachweisgrenzen und Temperaturabhangigkeit Bernd Brinkmann und Jurgen Bruns Institut fur Rechtsmedizin der Universitat Hamburg, Butenfeld 34, D-2000 Hamburg 54, Bundesrepublik Deutschland Typing ACP Isoenzymes in Blood Stains Testlimits and Temperature Dependancy Summary. Two series of test blood stains of known red-cell acid phos- phatase types A, AB, AC, B, BC, and C are applied to two different types of stain carriers (glass and cotton) and stored at different temperature ranges: (a) 37°C at high air humidity, (b) 22°C, (c) 4°C, and (d) -20°C. Electro- phoresis is carried out either on cellulose-acetate-foils or on thin agarose layers. Visualisation of the isoenzyme sites is achieved using umbelliferyl phosphate as a substrate. The most important results are: Electrophoresis on Cellogel-foils turns out advantageous compared to agarose since (a) less stain material is required, (b) reproducibility is rather reliable, and (c) electro- phoretic separation is clearer. Blood stains on glass are identified after longer periods of storage than stains on cotton. If stains are stored at room temper- ature the time limits of demonstration are in the range of 3 to 6 weeks. The size of the sample necessary for demonstrating varies widely. Approximately twenty to twenty-five per cent of a blood drop are needed. The method described is recommended as practicable and reliable. Key words: Blood stains, ACP-ACP isoenzymes in blood stains Zusammenfassung. Serien von frisch angelegten Blutspuren auf Glas und auf Baumwolle - alle sechs Phanotypen der sauren Erythrocytenphosphatase (acP) - werden verschiedenen Lagerungsbedingungen ausgesetzt: 37 C, hohe Luftfeuchtigkeit; 20°C, Raumtemperatur; 4°C, Kuhlschrank; -20°C, Tief- ktihlschrank. Die elektrophoretische Darstellung erfolgt parallel mit Hilfe der ,Cellogel-Elektrophorese sowie mit Hilfe der Agarose-Dunnschicht-Elektro- phorese. Die Darstellung der Isoenzyme erfolgt mit Hilfe des fluoiogenen Substrats 4-Methyl-Umbelliferylphosphat. Die wichtigsten Ergebnisse sind: Es werden zur acP-Typendifferenzierung nur Bruchteile eines Blutstropfens verwendet. Die Verwendung der ,,Cellogel- Technik ermoglicht a) geringen Spurensubstanzverbrauch, b) sichere Typen- differenzierung, c) langere Nachweisgrenzen (im Vergleich zur Agarose-Tech- nik). Spuren auf Glas lassen sich langer nachweisen als Stoffspuren. Die ACP=englische Abkurzung fur Acid Phosphatase 0044-3433/79/0084/0001/$1.20

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